PCR inhibited by Redivue nucleotides???

Alexander Kraev kraev at bc.biol.ethz.ch
Wed Oct 9 04:50:46 EST 1996


In article <k.grimaldi-ya023060040810961515210001 at nntp-server.bcc.ac.uk>,
k.grimaldi at ucl.ac.uk wrote:

> I recently came across a piece which states that RediVue nucleotides
> (Amersham) inhibit taq polymerase - does any body have any experience of
> this?

<snip>
It has just come to our attention, however, that Redivue
> nucleotides
> cannot be used in PCR reactions employing Taq DNA Polymerase. The substance
> added to Redivue to stabilize the compound at higher temperatures longer is
> severely
> inhibitory to this enzyme. This fact is not divulged in the product insert
> literature. If
> your application calls for radiolabeling of nucleic acids by PCR, using
Taq DNA
> Polymerase, choose a standard, non Redivue, 32-P labeled nucleotide.

We have not used Redivue for probe labelling with Taq, but we routinely
used gamma-ATP for cycle sequencing. Up to 15 uCi in a total of 25 ul have
no ill effect on the result, although the reactions are visibly rosy coloured.


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