mac446 at hastingslab.harvard.edu (Thomas F. Fagan) wrote:
>Isn't it a matter of unfolding? The more concentrated the enzyme is the
>more likely it is to be bound to other enzyme molecules by hydrogen
>bonding or other interactions and the less likely it is to spontaneously
>unfold. This is why you can substitute "enzyme" with BSA or some other
>stabilizing protein.
Yep, I agree - the purified proteins (it happens with antibodies too)
just get lonely, and inactivate - by whatever mechanism.
Pete
