Why are concentrated enzymes more stable?

Pete bss3pk at surrey.ac.uk
Tue Oct 8 10:55:50 EST 1996


mac446 at hastingslab.harvard.edu (Thomas F. Fagan) wrote:

>Isn't it a matter of unfolding? The more concentrated the enzyme is the
>more likely it is to be bound to other enzyme molecules by hydrogen
>bonding or other interactions and the less likely it is to spontaneously
>unfold.  This is why you can substitute "enzyme" with BSA or some other
>stabilizing protein.

Yep, I agree - the purified proteins (it happens with antibodies too)
just get lonely, and inactivate - by whatever mechanism.

Pete




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