Low copy protocol

Rafael Maldonado rafael at host.med.utah.edu
Mon Oct 7 12:45:48 EST 1996


On 4 Oct 1996, Lou Cantolupo wrote:

> Does anyone have a good protocol for isolating low copy # pDNA from
> bacteria?

Follow the same protocol for high copy, increasing the volumes of 
culture ans solutions. Try a purification step at the end as CsCl 
centrifugation or PEG precipitation, because the DNA will be dirtier 
and more contaminated with chromosomal DNA (just because you end up 
with more of everything...)

Rafa

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