On 4 Oct 1996, Lou Cantolupo wrote:
> Does anyone have a good protocol for isolating low copy # pDNA from
> bacteria?
Follow the same protocol for high copy, increasing the volumes of
culture ans solutions. Try a purification step at the end as CsCl
centrifugation or PEG precipitation, because the DNA will be dirtier
and more contaminated with chromosomal DNA (just because you end up
with more of everything...)
Rafa
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Rafael Maldonado | 'No te creas todo
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University of Utah |
Salt Lake City, Utah 84112. USA. | "Don't believe
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