In article <325D9AD9.32DE at GENVEC.COM>,
CANTOLUPO at GENVEC.COM (Lou Cantolupo) wrote:
->Well, I took it upon myself to call the centrifuge experts, Beckman.
Wow, what a rigor!
->HOWEVER. In an ultra centrifuge, where you routinely approach speeds in
->excess of 80,000 rpm (as in CsCl grad's), it is imperetive that you fill
->all slots with equal weights. In ultra, where the rotor tolerance is
->5/10,000 of an inch in the eliptical orbit, equal vector forces prove
->not to be so equal unless they are 100% equal. In otherwords, when you
->go fast, the rules can't be bent as easily; you have to optimize your
Yep. That's exactly why for [ultras AND big rotors] it is highly
recommended that one always fills all positions with ~ equal weights. Yes,
this can be irritating on a 8-positions when all you need is a single
gradient, but good care of ultracentrifuge and - particularly - rotor
implies just that.
Off to for a spin,