sequence is blurry

[jose manaligod md]

In article <genecutl-0410961813020001 at kosh-161b-3.berkeley.edu>,
genecutl at mendel.berkeley.edu (gc) wrote:

> I've been having trouble reading my sequencing gels because the
> bands tend to get blurry after about 60-100 bases from the bottom
> and I can't tell if I'm looking at single bands or doublets.  I've 
> been running 5% acrylamide gels at 35-40 watts.  The buffer is 
> 1xTBE, except that I add sodium acetate to 1M in the bottom buffer
> after the gel has run about half way.  Any suggestions on sharpening 
> my bands would be grealy appreciated.
> 
> --gc
> TheRoadToNowhere
> http://sp1.berkeley.edu/

I have successfully used FMC Long Range Gel solution which gives very
clear bands and a much longer reading of the base pairs.