sequence is blurry
jose manaligod md
jmanalig at blue.weeg.uiowa.edu
Thu Oct 10 14:45:51 EST 1996
In article <genecutl-0410961813020001 at kosh-161b-3.berkeley.edu>,
genecutl at mendel.berkeley.edu (gc) wrote:
> I've been having trouble reading my sequencing gels because the
> bands tend to get blurry after about 60-100 bases from the bottom
> and I can't tell if I'm looking at single bands or doublets. I've
> been running 5% acrylamide gels at 35-40 watts. The buffer is
> 1xTBE, except that I add sodium acetate to 1M in the bottom buffer
> after the gel has run about half way. Any suggestions on sharpening
> my bands would be grealy appreciated.
I have successfully used FMC Long Range Gel solution which gives very
clear bands and a much longer reading of the base pairs.
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