Sonication

[The Kindly One]

>I always do my sonication on 3-5 ml (in Corex 15 tubes) (corresponding to
>400ml culture).
>Sonicate 8 x 15 sec with 30 sec on ice between. Lysis was estimated to 90
>percent (estimated on the amount of released overexpressed protein).
>
Thanks to the many wh have responded, all with similar answers. What
I need now is what POWER to use!!! The machine I'll be using only
grades in %, but I need to know what wattage? ti use!!!

Thanks again
Scott

-- 
** Scott D. McCulloch(Bunny Lover)	| If we knew what we were doing,
** smccull at jurassic.fisher.calpoly.edu	| it would not be called     
** BioChemical Effects Research		| research, now would it?
** http://www.calpoly.edu/~smccullo/    |