Sonication

Pascal Mertens Pascal.Mertens at fundp.ac.be
Thu Oct 10 10:51:04 EST 1996


In article (Dans l'article) <53e931$rn at isnews.csc.calpoly.edu>,
smccull at jurassic.fisher.calpoly.edu (The Kindly One) wrote (écrivait) :

> Can someone suggest a sonication treatment necessary to lyse E. coli 
> that's overexpressing a protein? It would be for 20-50 mL of
> solution containing cells from 1-2 L of culture.

I always do my sonication on 3-5 ml (in Corex 15 tubes) (corresponding to
400ml culture).
Sonicate 8 x 15 sec with 30 sec on ice between. Lysis was estimated to 90
percent (estimated on the amount of released overexpressed protein).

Hope this helps.
Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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