Here are some references to support my previous reply: Bauer et al, Nucleic Acid research, 21(18):4272, 1993. Lohmann, Biotechniques, 18(2):200, 1995. Liang et al, Nucleic Acid Research, 22:5763, 1994. Trentmann et al, Science, 267:1186, 1995 (see response by Liang).