Re-PCRing after gel-purification problems

Bill Burnett bbur at wpo.nerc.ac.uk
Thu Oct 10 10:35:06 EST 1996


barry at sccs.swarthmore.edu (Brendan) wrote:
>Hi,
>
>   I'm having trouble simply Re-PCRing products after gel purifying them. 
>I use TAE gels and SpinX columns to gel purify first round bands.  Why is
>this second round not working?  Does PCR work with templates that are in
>Acetate (tAe)?  Any ideas....I'm stumped.
>
>Barry Mook

Hi Barry... the E in your TAE will tie up Mg2+ ions... could that be the 
problem?

B.
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Bill Burnett: bbur at dml.ac.uk            
Scottish Association for Marine Science
P.O. Box 3, Oban, Argyll, PA34 4AD.





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