PNA RAS PCR

Graham Atherton grggta at picr.cr.man.ac.uk
Thu Oct 10 03:19:39 EST 1996


Has anyone used PNA oligomers to amplify pure population of PCR
DNA from a mixed population of cells containing mutant and wild-
type RAS oncogene i.e. to amplify the mutant (activated)RAS allele
from a clinical specimen containing say 5% tumour cells and
95% normal cells. This would then provide a suitable template to 
sequence the RAS oncogene in tumour cells.
Thanks for any help.
Graham Atherton     



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