Has anyone used PNA oligomers to amplify pure population of PCR
DNA from a mixed population of cells containing mutant and wild-
type RAS oncogene i.e. to amplify the mutant (activated)RAS allele
from a clinical specimen containing say 5% tumour cells and
95% normal cells. This would then provide a suitable template to
sequence the RAS oncogene in tumour cells.
Thanks for any help.
Graham Atherton
