Site directed mutagenesis

Florence CABON cabon at infobiogen.fr
Fri Oct 11 11:48:08 EST 1996


By gel retardation (EMSA), I have identified a sequence implicated in
the regulation of the gene I'm studying. I have a reporter vector
containing this promoter and I want to introduce a mutation in this
vector. I  am puzzled by the number of commercial kits for that. As all
claim magic 99% results in 5 min or so :-) I do not know how to choose. 
What about PCR in that purpose ?
I have 22 mers oligos corresponding to the wild type and mutated
sequence.
Please Help !

 
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Florence Cabon. CNRS Villejuif, France. cabon at infobiogen.fr
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