cloning

Karyn Esser mlc25 at uic.edu
Fri Oct 11 11:53:27 EST 1996


We are having trouble cloning  a 250bp blunt-sticky (EcoR1) fragment
into a vector cut with Sma and R1.  Does anyone have any advice for
ligation conditions?

One problem may be with blunting an Sph end with T4 DNA polymerase.  Are
there any recommendations for that?

Thanks

Karyn



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