In article <Pine.LNX.3.91.961009171722.8186A-100000 at morpheus.wustl.edu>,
gerry at MORPHEUS.WUSTL.EDU (Gerald Downes) wrote:
>> I received a pamplet from Clontech that describes their
> pZErO cloning vector. Of course it *sounds* like heaven on earth (ease
> of use with no background) but is there a catch? Does anyone have
> experience with this vector or another that is supposed to give
> virtually no backgroud (CIP treated vectors excluded).
If you follow their directions, there's no catch! I have used this vector
many times to construct genomic libraries and I get at least 90% inserts.
It has saved me weeks of frustration trying to optimize vector/insert
ratios and/or dealing with alkaline phosphatase.