pZErO from Clontech

Steven Goldberg goldberg at bms.com
Fri Oct 11 10:00:09 EST 1996


In article <Pine.LNX.3.91.961009171722.8186A-100000 at morpheus.wustl.edu>,
gerry at MORPHEUS.WUSTL.EDU (Gerald Downes) wrote:

> Netters!
> 
> I received a pamplet from Clontech that describes their 
> pZErO cloning vector.  Of course it *sounds* like heaven on earth (ease 
> of use with no background) but is there a catch?  Does anyone have 
> experience with this vector or another that is supposed to give 
> virtually no backgroud (CIP treated vectors excluded).
> 

If you follow their directions, there's no catch!  I have used this vector
many times to construct genomic libraries and I get at least 90% inserts. 
It has saved me weeks of frustration trying to optimize vector/insert
ratios and/or dealing with alkaline phosphatase.

Good luck.

Steve



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