We have been trying to clone 50-800bp genomic DNA fragments which have
poly-Purine/ poly-Pyrimidine stretches. The clones (in psk+) are very
unstable and throw out the inserts. Could anybody suggest suitable
vectors/host strains to solve this problem.
Please send the replies directly to me, apart from the the group.
Thanks in advance.
Aditya K. Sengupta
Developmental Biology and Genetics Laboratory,
Indian Institute of Science,
Bangalore 560 012, India.
email aditya at serc.serc.iisc.ernet.in