cloning highly methylated DNA
Aditya K Sengupta
aditya at serc.serc.iisc.ernet.in
Fri Oct 11 05:05:30 EST 1996
We are facing considerable difficulty in cloning highly methylated DNA
from an insect genome source, for constructing a genomic library. This
genome is known to have Me-CpA, Me-CpG and Me-CpT from nearest neighbour
analysis. Could anybody suggest suitable vectors/host strains and/or
treatments to solve this problem.
Please email me the replies directly, apart from to the group.
Thanks in advance.
Aditya K. Sengupta
Developmental Biology & Genetics Laboratory,
Indian Institute of Science, Bangalore 560 012,
email aditya at serc.serc.iisc.ernet.in
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