cloning highly methylated DNA

Aditya K Sengupta aditya at
Fri Oct 11 05:05:30 EST 1996

 We are facing considerable difficulty in cloning highly methylated DNA 
from an insect genome source, for constructing a genomic library. This 
genome is known to have Me-CpA, Me-CpG and Me-CpT from nearest neighbour 
analysis. Could anybody suggest suitable vectors/host strains and/or 
treatments to solve this problem.
 Please email  me the replies directly, apart from to the group.
 Thanks in advance.

Aditya K. Sengupta
Developmental Biology & Genetics Laboratory,
Indian Institute of Science, Bangalore 560 012,
email  aditya at

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