tissue stored at -20 C -- RNA still OK?

[Mary Clare Tracey]

Juergen Brosius wrote:
> 
> If we do not use fresh tissue for RNA preps we store it prior to >prepping at -70 C and never thaw it in between.  I have the option to >use tissue of interest that, however, had been stored at -20 C.  Does >anyone have experience concerning the state of the RNA extracted from >such a source? I remember vaguely that a similar question was posted >before but I did not see the outcome.

Hi

I worked with human tissues which had been stored for some 
considerable time at -20°C before they were made available to me. I 
noticed some reduction in quantity of extractable RNA across all 
tissues and some reduction in quality in certain tissues. However, I 
still managed to get enough RNA for my Northerns and they worked okay 
(as well as Northerns ever do!).

Regards

MC


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Mary Clare Tracey		email: maryclareatkokako.wnmeds.ac.nz
Wellington     **NOTE: change the at to @ for reply. Avoiding spam!** 
New Zealand