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oligo gene assembly protocol?

AShaw5 ashaw5 at aol.com
Mon Oct 14 00:05:44 EST 1996


I am trying to assemble a gene fragment of 200bps by synthesizing it
completely using oligos.  I don't want to make 2 200mer oligos, so I have
made a set of overlapping oligos.  

Does anyone have a protocol for putting these together?  Do I worry about
kinasing them?  Do I heat and renature with all of them mixed together or
one by one?  

Any help with this is appreciated.
Andrey Shaw



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