A question on phage expression systems
rand at pelican.dbe.csiro.au
Mon Oct 14 01:40:16 EST 1996
In article <henry.12.000DEA04 at whio.lincoln.ac.nz>,
henry at whio.lincoln.ac.nz (Hayden Henry) wrote:
> Hi all! I'm writing an essay on phage expression of scFV (single chain
> antibody variable region fragments), and I have a question.
> The primary question is: Can the phage be correctly packaged in a
> bacteria, without ANY phage pillus?
> The background to this question is as follows:
> In the early papers e.g. McCafferty et al., Nature 348, 552-554, they
> inserted a synthetic sequence they wanted to express, into an fd-CAT1
> vector and interrupted the coat protein III gene (though if you look at the
> diagram it has incorrectly been labelled a coat protein VIII gene!). The
> idea was that they could transfect this into an E.coli and express a phage
> with the scFV attached on the phage surface.
I see that someone's already answered your questions, but I thought I
should defend the McCafferty paper - the diagram looks correct to me, and
the confusion is probably because gene VIII and gene III are close
Keith Rand, Sydney Australia
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