help for blunting 5' and 3' overhangs

Kenneth Y. Ilio you at somehost.somedomain
Mon Oct 14 13:21:20 EST 1996


I am trying to modify a gene by blunting the ends of Pst1 (gives 5' 
orverhang) and BamH1 (gives 3' overhang). We have tried to blunt the ends 
by using T4 polymerase to chew up the ends of Pst1 site and fill in the 
3' overhang of BamH1. However, we had no luck. Does anyone have any 
suggestions for us to try? Please specify the enzymes that you have used. 
You can post the answer on the net. Thank you.

Isaac.



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