Benzamidine Sepharose

DM. Henderson bijgh at zeus.bris.ac.uk
Mon Oct 14 06:24:16 EST 1996


Hi,

I've cleaved the His.Tag off my protein and now I want to remove thrombin 
from the solution.  Has anybody got a protocol for using a slurry of 
benzamidine sepharose instead of having to set up a column?  I was 
thinking of throwing in a bit of benzamidine-seph, shaking a bit, then 
spinning it out.  Is it really that simple?

Cheers,

Jared  

-- 
Jared Head     at the Department of Biochemistry, University of Bristol




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