I've cleaved the His.Tag off my protein and now I want to remove thrombin
from the solution. Has anybody got a protocol for using a slurry of
benzamidine sepharose instead of having to set up a column? I was
thinking of throwing in a bit of benzamidine-seph, shaking a bit, then
spinning it out. Is it really that simple?
Jared Head at the Department of Biochemistry, University of Bristol