I need a bacterial plasmid that has a ts origin of replication or some
other mechanism such that when placed at the non-permissive temp. it no
longer remains in the cell. I currently use a plasmid, pPM103, a ts
derivative of pSC101, but I found that it is very leaky at the
non-permissive temperature. I do not know of any commercially available
ts plasmids, but if they exisits, I could certainly buy one of those.
Any Help Would be Appreciated.
Jeff Huckaby Jeff Huckaby
Department of Biology 426 OML
email huckabyj at minerva.cis.yale.edu Yale University
office:(203) 432-3877 New Haven, CT 06511
WWW http://www.yale.edu/~huckabyj/topbio.html Lab:(203) 432-3982