recirculating electrophoresis buffer

[Lou Cantolupo]

The big argument is that you'll get a conductivity difference between
the two sides--as the gel runs, more ions acumulate on the negative (?)
side of the box and your gel will run funny.

I never recirc my buffer, even for long runs (3+ hours).  Some will
argue that for Northerns you have to, but I never do that either.  

Lou Cantolupo
GenVec, Inc.
Dept. Molecular Virology
Rockville, MD  20852
CANTOLUPO at GENVEC.COM