Mitochondria Isolation

[Delvac Oceandy]

I have problem in isolating mitochondria from mammalian tissues (e.g. liver,
kidney, muscle)
My problem is in the homogenization of the tissue in isolation buffer 
If somebody has experience in isolating mitochondria, please tell me the
best method to homogenize the tissue. 
Which one is better, using motor-driven homogenizer or hand operated
homogenizer, to get a good yield and purity of mitochondria ??

Delvac Oceandy
Eijkman Institute 
Jakarta Indonesia
<delv at eijkman.go.id>