Jeff Huckaby wrote:
>> Netters
> I need a bacterial plasmid that has a ts origin of replication or some
> other mechanism such that when placed at the non-permissive temp. it no
^^^^^^^^^^^^^^^
> longer remains in the cell.
Snip
Any Help Would be Appreciated.
Track down a PolI mutant of E. coli. If my memory serves me right, colE1-based
plasmids require PolI for replication and the ts mutants of PolI (dnaA-?), when raised
to the none-permissive temperature, will "cure" the cell of the plasmid. BTW, all
pBR322 (aka pUC) vectors are derived from colE1-am I right?
Hope this helps.
Jim Miller
Lilly Research Labs
jrm at lilly.com
> Thanks
> Jeff
>> ==============================================================================
> Jeff Huckaby Jeff Huckaby
> Department of Biology 426 OML
> email huckabyj at minerva.cis.yale.edu Yale University
> office:(203) 432-3877 New Haven, CT 06511
> WWW http://www.yale.edu/~huckabyj/topbio.html Lab:(203) 432-3982
> ==============================================================================
