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Frozen restriction enzymes

Michael Sachsamanoglou michaels at PHARM.MED.YALE.EDU
Tue Oct 15 04:02:38 EST 1996

Hi Netters,

Here is my quick question:
What happens when a restriction endonuclease solution is frozen to -80C?
Is it normal for the 50% glycerol storage solution to solidify at this 
temperature? I've heard sometime ago that if a restriction enzyme 
freezes it is dead. Is this true? Thanks for any answers.


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