PRINS / midisat

[Manfred Schuermann]

Primed In Situ (PRINS) labeling is an interesting method to identify 
specific chromosome regions. This technique works well and easily with 
centromeres and 1p36 midisatellite DNA. So now I am looking for human 
DNA sequences with a lower degree of reiteration that are

          i) present with some 20 (or more) copies of a >20 bp motif
         ii) evenly spaced over 20 kb or more
        iii) clustered at one or few loci in the human genome. 

Allthough the protein structure is quite repetitive and the gene is 
distributed over many exons, *collagenes* (and others) turned out to be 
much too variable on the DNA level. The recombination signal region of the 
*immunoglobulin* genes could be a good candidate, but the string of perfect
match is only 8 or 9 bp long.

Does anybody out there have a good idea where else to look?

Thank you very much for your support!


Manfred Schuermann, M.D.

   Institute of Human Genetics
   University of Luebeck Medical School, Germany  
   
         e-mail   msms at on-luebeck.de
         phone    +49 451 500 2620
         fax      +49 451 500 4187