Primed In Situ (PRINS) labeling is an interesting method to identify
specific chromosome regions. This technique works well and easily with
centromeres and 1p36 midisatellite DNA. So now I am looking for human
DNA sequences with a lower degree of reiteration that are
i) present with some 20 (or more) copies of a >20 bp motif
ii) evenly spaced over 20 kb or more
iii) clustered at one or few loci in the human genome.
Allthough the protein structure is quite repetitive and the gene is
distributed over many exons, *collagenes* (and others) turned out to be
much too variable on the DNA level. The recombination signal region of the
*immunoglobulin* genes could be a good candidate, but the string of perfect
match is only 8 or 9 bp long.
Does anybody out there have a good idea where else to look?
Thank you very much for your support!
Manfred Schuermann, M.D.
Institute of Human Genetics
University of Luebeck Medical School, Germany
e-mail msms at on-luebeck.de
phone +49 451 500 2620
fax +49 451 500 4187
