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Long PCR primers

Joe Miano jmiano at post.its.mcw.edu
Tue Oct 15 08:46:34 EST 1996


If you do choose to PCR with such a monster primer, I would go the extra
expense and have the FULL length oligo gel purified.  As you may know the
coupling efficiency of the most 5' bases will not be great (even if the
efficiency is 99%/cycle, which few oligo synthesizers are, by the time you
get out to your c-myc tag, the efficiency will be less than 30%; in other
words, greater than 70% of your oligo yield will be truncated).  

Best of luck and please post your results!

Joe Miano
The Love you take is equal to the Love you make--P. McCartney

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