CML TR-PCR

Herbert Ludewick 075bert at chiron.wits.ac.za
Tue Oct 15 12:39:25 EST 1996


WE ARE CURRENTLY EXPERIENCING DIFFICULTY WITH OUR RT-PCR TO DETECT CML.
WE ARE RT 0.25ug OF RNA AND USING PCR PRIMERS DESCRIBED IN JUERGEN MAURER 
PAPER PUBLISHED IN BLUT(1990).

WE NORMALLY HAVE TO REPEAT OUR PCR SEVERAL TIMES. INTEGRITY OF RNA IS 
OCCASSIONALLY CHECKED.

DOES ANYONE HAVE A MORE ROBUST METHOD TO DETECT THE P210 AND P190.

DESPERATELY SEEKING HELP.

HERBERT.



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