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lysing of fixed cells

Diane Judd d.judd at ic.ac.uk
Tue Oct 15 08:19:12 EST 1996

I would like to know is it possible to lyse fixed cells effectively? And if
so, how? 

I am trying to ascertain how fixation (4% formaldehyde) effects the
fluorescence of a particular protein in cos7 cells and therefore want to
lyse them after fixation in order to measure fluorescence of the lysate in a


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