Sonication

Pascal Mertens Pascal.Mertens at fundp.ac.be
Tue Oct 15 08:10:20 EST 1996


In article (Dans l'article) <53jfav$pcf at isnews.csc.calpoly.edu>,
smccull at jurassic.fisher.calpoly.edu (The Kindly One) wrote (écrivait) :

> >I always do my sonication on 3-5 ml (in Corex 15 tubes) (corresponding to
> >400ml culture).
> >Sonicate 8 x 15 sec with 30 sec on ice between. Lysis was estimated to 90
> >percent (estimated on the amount of released overexpressed protein).
> >
> Thanks to the many wh have responded, all with similar answers. What
> I need now is what POWER to use!!! The machine I'll be using only
> grades in %, but I need to know what wattage? ti use!!!

Use the max allowed power for your device (70 watts in my case, with a
sonicating tip).

Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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