In article <961014142939.2e612f87 at AZCC.Arizona.EDU>,
JMARTINEZ at AZCC.ARIZONA.EDU wrote:
> How fragile is phosphocellulose? I need to prepare a phosphocellulose column
> as part of a fractionation protocol and would like to calibrate it with some
> test mixtures before I put the real stuff on. However, if I interpret
> Whatman's product literature phosphocellulose cannot be left in aqueous
> for any length of time. Is this correct? What do you chromatography experts
> do when using phosphocellulose? Any feedback would be appreciated.
When I use to work with phosphocellulose, I would make it up in large
batches and store in 0.5 M Na-Phosphate and 1 M NaCl. I never re-used the
column because it would get compacted and give poor flow rates.