Q: polyA tail length vs. mRNA capture
sullivan at gwis2.circ.gwu.edu
Tue Oct 15 18:18:44 EST 1996
the length of polyA tails on various mRNAs is known to change in both
directions during early development in many organisms. This raises two
similar questions pertinent to my research:
1) anyone know what the *shortest* length of polyA is that gets captured on
oligo dT columns or hybridized to oligodT primers (e.g. LTI's oligo dT12-18)?
2) does reverse transcription pick up mRNAs with short polyA tails (and
if so how short)?
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