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Q: polyA tail length vs. mRNA capture

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Tue Oct 15 18:18:44 EST 1996

the length of polyA tails on various mRNAs is known to change in both 
directions during early development in many organisms.  This raises two 
similar questions pertinent to my research:

1) anyone know what the *shortest* length of polyA is that gets captured on 
oligo dT columns or hybridized to oligodT primers (e.g. LTI's oligo dT12-18)?

2) does reverse transcription pick up mRNAs with short polyA tails (and 
if so how short)?

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