Aminoglycoside resistance

Peter Wang plw at mrc-lmb.cam.ac.uk
Tue Oct 15 19:06:49 EST 1996


Dear Jose:

jose.mejia at well.ox.ac.uk wrote:
> I need to know if there are any aminoglycosides inactivated by the type I
> phosphotransferase encoded on Tn903, which are not inactivated by the type
> II enzyme from Tn5. Or the reverse.

Check out the following review:

Shaw KJ, Rather PN, Hare RS, Miller GH.  1993.  "Molecular genetics of 
aminoglycoside resistance genes and familial relationships of the 
aminoglycoside-modifying enzymes." Microbiological Reviews 57(1):138-163

According to it, the gene from Tn903 is classified as aph(3')-Ia and provides 
resistance to kanamycin, neomycin, paromomycin, ribostamycin, lividomycin, and 
gentamicin B. The gene from Tn5 is classified as aph(3')-IIa and provides 
resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and 
gentamicin B. So it would seem that lividomycin and butirosin might distinguish the 
two enzymes.

Cheers,
- Peter

---------------------------------------------------------
Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
---------------------------------------------------------



More information about the Methods mailing list