pBS max insert size

[John Watson]

FABIAN MOEBIUS wrote:

> I need to sublone a 14 kb fragment. Is 10 kb an absolute maximum for the insert size for pBS? Is 
> someone aware of a plot between insert size and pBS/pBS-like plasmids cloning effiency?

I've cloned bigger stuff than 10 kb, but I have no idea what the "maximum" is.  Most recent big guy 
here is on the order of 20 kb.
----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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"If you're not part of the solution, you're part of the precipitate."
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