Re-PCRing after gel-purification problems

[John Watson]

Richard P. Grant wrote:

> We routinely use QIAEX to purify from TBE/agarose with no ill effects.
> However, given that I'm currently in the midst of a large project, this would
> have been expensive for my 2-round PCR expts.  So I just cut the bands out of
> a gel, melted, took 5 - 10 ul of the agarose and plonked it straight into a 50
> ul PCR rxn.  Superb.

I second this approach.  We use it in my lab all the time, though the volumes are 
more like 2-5 ul.

----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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"If you're not part of the solution, you're part of the precipitate."
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