Staph aureus RNA isolation and other questions

Susan Boyle-Vavra sboyleva at midway.uchicago.edu
Wed Oct 16 10:56:22 EST 1996


Anyone have a great RNA prep for S. aureus? Do the ribosomal bands have to be tight to get a good signal from a message in low copy?
We have tried Trizol with a Phenol step but the RNA 
doesn't stay stable at -70.

Any ideas about how to lyse S. aureus w/o lysostaphin and that works better than sonication??
Does anyone know the mechanism of achromopeptidase lysis?

Thanks.
Susan




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