recirculating electrophoresis buffer

[Manuel G. Claros]

On Sun, 13 Oct 1996, Lely Chow wrote:

 > Can anyone tell me what happens if one does not recirculate
 > electrophoresis buffer after running an agarose gel for an extended amount
 > of time?

A pH gradient is generated. Specially important for RNA EF. DNA buffers
are more "buffering" and less sensible. See Maniatis or current Protocols
for more details.

Manuel G. CLAROS, Ph.D.
Bioq. & Biologia Molecular
Fac. Ciencias
Campus de Teatinos
E-29071 Malaga