recirculating electrophoresis buffer
Manuel G. Claros
claros at orgiva.cica.es
Wed Oct 16 03:59:10 EST 1996
On Sun, 13 Oct 1996, Lely Chow wrote:
> Can anyone tell me what happens if one does not recirculate
> electrophoresis buffer after running an agarose gel for an extended amount
> of time?
A pH gradient is generated. Specially important for RNA EF. DNA buffers
are more "buffering" and less sensible. See Maniatis or current Protocols
for more details.
Manuel G. CLAROS, Ph.D.
Bioq. & Biologia Molecular
Campus de Teatinos
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