A few questions :
1. Are there any consensus sequences for 5' and 3' intron splice sites,
and if so, what are they?
2. Is there a known minimum intron length?
3. Will hairpin loops (such as the T7 terminator) disrupt EUKARYOTIC
transcription/translation to a great extent?
Here's were all this is going : could any disruption of translation be
ameliorated by placing such a structure between intron splice sites?
Thanks for any info!