Bapping

[SARAH LOUISE RAWLINGS]

Hello,

I was wondering if anybody could help me with my molecular bioligy 
problem. 
I am a student carrying out some reaserch into a protien phosphatase. I 
am trying to insert my DNA which has been blunted with Klenow into a 
vector that hes been cipped with Calf intestinal Phophatase. Upon trying 
to religate the DNA with my vector they do not ligate very easily (if at 
all)...I was wondering if anyone had any ideas on what the problem could 
be.This procedure has been repeated many times with the same lack of 
results.
The procedure being followed is that in Maniati's and the chemicals being 
used are not at fault. Thanks. 

Sarah Louise Rawlings       
Bsc Biochemistry (coventry University)
SLR2 at coventry.ac uk