need: info on A260-A280 absorbance measurements
Manuel G. Claros
claros at orgiva.cica.es
Wed Oct 16 09:08:36 EST 1996
On 14 Oct 1996, Guido Hooiveld wrote:
> I was told an A280/A260 ratio
> theoretically limit is 2.0. However, I often find a ratio larger than 2.0; like 2.1 to 2.2. So,
> is this (theoretically) value of 2.0 correct, if yes, does anybody have a suggestion why I find
> values >2; if not, what is this value?
You have degradated nucleotides.
> multiply A260 with 50 to obtain the concentration (ug/ml), or use 35? Which value is correct?
50 for oligonucleotides and 35 for dsDNA
> Any comments would be appreciated!
I hope this helps
Manuel G. CLAROS, Ph.D.
Bioq. & Biologia Molecular
Campus de Teatinos
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