IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

(no subject)

Helen Haase h.haase at mailbox.uq.oz.au
Wed Oct 16 22:37:48 EST 1996

I've been successfully doing Northerns for some time looking at mRNAs 
which range in size from 1-12kb.  The largest mRNA (12kb) has the lowest 
expression level.  Recently I lost the ability to detect this message.  
Using newly prepared probe (both cDNA and riboprobe) did not change the 
situation.  I now suspect that the transfer procedure from gel to 
membrane is to blame.  However I have not altered my protocol, which is 
to do a capillary blot O/N from 10XSSC to a positively charged nylon 
membrane (Boehringer Membrane).  All other targets I look for are 
present in expected quantities.  Has anyone else experienced problems 
with transfer of higher molecular weight RNA?  If so what are your 

Science is such fun!!

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net