Why is sacB expression lethal in E. coli?
somewhere at universe.com
Wed Oct 16 20:46:50 EST 1996
Product of enzymatic conversion of sucrose by SacB causes death in many
gram-negatives by osmotic shock (cells burst).
REWH Lab <rewhlab at cbdn.ca> wrote in article <53udca$jl1 at nntp.ucs.ubc.ca>...
> I can't find any reference to why sacB functions as a
> counterselective marker in E. coli in the presence of 5-10% sucrose.
> anyone know why it works?
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