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Why is sacB expression lethal in E. coli?

someone somewhere at universe.com
Wed Oct 16 20:46:50 EST 1996

Product of enzymatic conversion of sucrose by SacB causes death in many
gram-negatives by osmotic shock (cells burst).


REWH Lab <rewhlab at cbdn.ca> wrote in article <53udca$jl1 at nntp.ucs.ubc.ca>...
> Hi,
> 	I can't find any reference to why sacB functions as a 
> counterselective marker in E. coli in the presence of 5-10% sucrose. 
> anyone know why it works?

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