Bapping

[Gene Huh]

In article <Pine.ULT.3.91.961016102221.18991M-100000 at rowan>, SARAH LOUISE
RAWLINGS <slr2 at coventry.ac.uk> wrote:

# Hello,
# 
# I was wondering if anybody could help me with my molecular bioligy 
# problem. 
# I am a student carrying out some reaserch into a protien phosphatase. I 
# am trying to insert my DNA which has been blunted with Klenow into a 
# vector that hes been cipped with Calf intestinal Phophatase. Upon trying 
# to religate the DNA with my vector they do not ligate very easily (if at 
# all)...I was wondering if anyone had any ideas on what the problem could 
# be.This procedure has been repeated many times with the same lack of 
# results.
# The procedure being followed is that in Maniati's and the chemicals being 
# used are not at fault. Thanks. 
# 
# Sarah Louise Rawlings       
# Bsc Biochemistry (coventry University)
# SLR2 at coventry.ac uk 

Sarah,

You may want to supply a few more details.  Do you mean you have no
colonies at all?  What controls were run?

-- 
Gene S. Huh, Ph.D.
Department of Molecular and Cell Biology
Life Sciences Addition, Room 221
University of California at Berkeley
Berkeley, California   94720-3200