As a minor part of my research project, I isolated total RNA from
estradiol-treated and control cells followed by RT-PCR. This was done
to compare the mRNA levels of a certain gene. Since the resultant
bands seemed of equal intensity following agarose gel electrophoresis
and ethidium bromide staining, I stated that the estradiol treatment
did not affect the mRNA levels. In retrospect, I know I should have
done a northern, but was this conclusion still acceptable?