In article <326366E7.5A88 at biologie.uni-ulm.de>,
volker.knoop at biologie.uni-ulm.de wrote:
> Hi, there,
> Anybody having a good and detailed reference concerning the use of
> different salts and alcohols for differential precipitation of DNA and
> RNA (more than in Maniatis, 2nd)? I read a good summary somewhere
> sometime but I forget..
> Most importantly, we routinely separate DNA and RNA in a first step
> with 2 M Lithium acetate - and now I need the reference for this!!
> Thanks for help
In the book "Fundamental Techniques in Virology", from about 1970 or so,
there was a description of using LiCl precipitation to precipitate RNA.
You might also look at Aufrey and Rougeon, Eur. J. Biochem 107:303-314,
1980 "Purification of mouse immunoglobulin heavy-chain messenger RNAs from
total myeloma tumor RNA".
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