lysis problems with bacteriophage cultures

Michelle Gleeson michelle at MOLECULE.BIO.UTS.EDU.AU
Thu Oct 17 18:35:40 EST 1996


Hi Helene,

Have you tried the alternative strain suitable for this kit, LE392?  I
have had no problems with it.  Good luck

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Michelle Gleeson                      Thought for the day...
Molecular Parasitology Unit     Remember that the toes you step on today
University of Technology        could well be attached to the legs that
Sydney AUSTRALIA                support the arse you need to kiss tomorrow
michelle.gleeson at uts.edu.au
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On 17 Oct 1996, Artur da Camara Machado wrote:

> Hallo,
> has anyone an idea why bacterial cultures (KW251) inoculated with lambda-=
GEM11
> do not lyse after overnight incubation at 37=B0C. The small scale liquid =
culture of phages
> was performed according to Maniatis et al.  0.1 ml bacteria (grown overni=
ght in NZY
> medium + 0.2% maltose) in 10mM MgSO4 were incubated with 0.1 ml bacteriop=
hage
> suspension in SM buffer for 15min at 37=B0C. Then 2ml prewarmed NZY mediu=
m were
> added and the culture was grown overnight at 37=B0C. But no lysis occurre=
d !!!
> Neither addition of an equal vol. of medium and further incubation for 3h=
 nor incubation
> at elevated temperature (39=B0C =3D optimum for lambda-phages to grow) co=
uld induce lysis.
> What is wrong with my cultures? Is the ratio bacteria : bacteriophages at=
 the initiation
> of the culture critical for complete lysis?
> I would be grateful for any help. Please, let me know your suggestions!
>
> Thanks, Helene.
>
>




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