In article <eettehad-161096215654 at sea-wa15-21.ix.netcom.com>,
eettehad at ix.netcom.com (Ellie Ettehadieh) wrote:
# I have been running PAGE for the past six years. I just moved to a new lab
# and today I made up all my solutions and tried making a polyarylamide gel
# to purify some DNA. To my surprise my gel would polymerize but it would
# look rather cloudy in a milky sort of way. I was not able to figure what
# the problem was. Using different solutions of acrylamide or buffer or even
# temed did not help. Had anyone out there ever experience such a thing?????
I've occasionally poured gels in which tiny bubbles have come out of
solution. Maybe you have *lots* of bubbles? If so, degassing before
pouring would help.
Gene S. Huh, Ph.D.
Department of Molecular and Cell Biology
Life Sciences Addition, Room 221
University of California at Berkeley
Berkeley, California 94720-3200