Cloudy PAGE.

[Gene Huh]

In article <eettehad-161096215654 at sea-wa15-21.ix.netcom.com>,
eettehad at ix.netcom.com (Ellie Ettehadieh) wrote:

# I have been running PAGE for the past six years.  I just moved to a new lab
# and today I made up all my solutions and tried making a polyarylamide gel
# to purify some DNA.  To my surprise my gel would polymerize but it would
# look rather cloudy in a milky sort of way.  I was not able to figure what
# the problem was.  Using different solutions of acrylamide or buffer or even
# temed did not help.  Had anyone out there ever experience such a thing?????
# 
# Ellie..

I've occasionally poured gels in which tiny bubbles have come out of
solution.  Maybe you have *lots* of bubbles?  If so, degassing before
pouring would help.

-- 
Gene S. Huh, Ph.D.
Department of Molecular and Cell Biology
Life Sciences Addition, Room 221
University of California at Berkeley
Berkeley, California   94720-3200