ligation of PCR products
kucera~gt at glaxo.com
Wed Oct 9 03:08:39 EST 1996
Zhiyong Zhao wrote:
> I wonder if it is possible to add phosphate group to the 5'-end in the primer s
> ynthesis reaction.
Someone correct me if I am wrong because I am going under
the assumption that if an oligo has been de-protected
following synthesis, it SHOULD contain a 5' phosphate group.
Oligos are synthesised in a 3'->5' direction, therefore, a
phosphate group Must be present on the last base added (i.e.
de-protected) before the next base is added.
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709
More information about the Methods