ligation of PCR products

Gary Kucera kucera~gt at glaxo.com
Wed Oct 9 03:08:39 EST 1996


Zhiyong Zhao wrote:
> 
> I wonder if it is possible to add phosphate group to the 5'-end in the primer s
> ynthesis reaction.


Someone correct me if I am wrong because I am going under 
the assumption that if an oligo has been de-protected 
following synthesis, it SHOULD contain a 5' phosphate group.  
Oligos are synthesised in a 3'->5' direction, therefore, a 
phosphate group Must be present on the last base added (i.e. 
de-protected) before the next base is added.
-- 
________________________________________________________
Gary T. Kucera, Scientist
Cellular Sciences/Transgenics Group
GlaxoWellcome Research, Inc.
Five Moore Drive
Research Triangle Park, NC 27709



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