We have been having difficulty with detection of a proteolytic fragment
of a protein by immunoblotting. The problem seems to be that the fragment
is easily washed off PVDF membranes. This may be due to unusual
hydrophobic/philic properties of the protein in question. We have tried
modifications of transfer protocols and other membranes but have not seen
any improvement. Has anyone had a similar experience or had any
experience with cross linking proteins to blot membranes?
Please respond by e-mail to davidmc at scripps.edu
