Hi,
We are currently working on promotors regulated by a response regulator in
a bacteria. We have to make some constructions with a pKK-type vector a
it's important for us to have a transcription terminator after our gene.
There is one terminator but it seems to be in the wrong polarity. So, my
question is:
Are the transcription terminator oriented, I mean, if there is one
terminator, does it act from 5' -> 3' AND also from 3' -> 5' ?
Thanks in advance...
Flore Bellefontaine
URBM, Namur, Belgium
flore.bellefontaine at fundp.ac.be
--
Flore Bellefontaine
Unité de recherche en Biologie Moléculaire
Facultés Universitaires de Namur, Belgium
flore.bellefontaine at fundp.ac.be
